【发布时间】:2022-10-19 21:30:22
【问题描述】:
我是该领域的新手,正在尝试运行 fastq 屏幕。我已经下载了 fastq 屏幕所需的所有软件包。
当我尝试运行它时,我得到了这个错误。
Reading configuration from '/home/ahmad/FastQ-Screen-0.15.2/fastq_screen.conf'
Aligner (--aligner) not specified. Did not find Bowtie/Bowtie2/BWA paths and/or index files
Please check: you have provided the full path to the aligner INCLUDING the executable filename
Please check: the specified genome indices comprises the full path AND the basename of the index files
See documentation for further details
Please adjust configuration.
我打开了 fastq_screen.conf 并将 bowtie2 的路径调整为 /usr/bin/bowtie2 /usr/share/man/man1/bowtie2.1.gz
基因组参考路径 /home/ahmad/FastQ_Screen_Genomes/Human
但仍然得到同样的错误。请问有什么帮助吗??
【问题讨论】: